Composite

Part:BBa_K539673:Design

Designed by: Shu-Han Chang   Group: iGEM11_NCTU_Formosa   (2011-09-27)

a RBS (B0032)+ilvC+a RBS (B0032)+ilvD with RNA thermometer+tetR+terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 3243
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4132
    Illegal AgeI site found at 611
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 197
    Illegal BsaI site found at 3029
    Illegal BsaI site found at 3286
    Illegal BsaI.rc site found at 1016


Design Notes

Built in two weak expressing RBSs, ilvC, ilvD, 37’celcius regulator RBS, tetR and terminator (Part:BBa_K539641 with Part:BBa_K332033). Both of ilvC and ilvD had been mutated to delet the function of RE site at the origin so that we can submit this part to the iGEM.


Source

Both of the ilvC and ilvD come from the Escherichia coli str. K-12 substr. MG1655. And the part which repress Ptet when the temperature up to 37'Celius is designed by 2010 iGEM NCTU_Formosa.

References